Electrophoretic Analysis (Tricine-SDS-PAGE) of Bovine Caseins
نویسندگان
چکیده
An alternative electrophoretic method that improves the separation, identification and relative quantification of bovine milk casein fractions and casein hydrolysates was developed. The method is based in the Tricine-SDS-PAGE technique while the relative quantification of milk proteins has been achieved by digital densitometry. The advantages of this procedure for milk proteins and milk protein hydrolysates are that bands are well separated and can be readily detected and quantified. In the new method, αs1-, αs2-, βand κ-casein and low molecular weight peptides (casein degradation products with high electrophoretic mobility) that cannot be usually detected by other electrophoretic systems are visualized as sharp bands. RESUMEN. “Un Método Electroforético mejorado destinado al Análisis de Caseína Bovina”. Ha sido desarrollado un método electroforético alternativo que mejora la separación, identificación y cuantificación relativa de caseína bovina y de hidrolizados de caseína. El método se basa en la técnica de Tricina-SDS-PAGE, en tanto que la cuantificación relativa de las proteínas lácteas fue realizada por densitometría digital. Una de las ventajas de este procedimiento para proteínas de leche y de sus hidrolizados es que las bandas se encuentran bien separadas y pueden ser fácilmente detectadas y cuantificadas. En el nuevo método, αs1-, αs2-, βy κ-caseína y los péptidos de bajo peso molecular (productos de degradación de la caseína con elevada movilidad electroforética), que no pueden ser habitualmente detectados por otros sistemas electroforéticos, son visualizados como bandas nítidas.
منابع مشابه
Tricine – SDS - PAGE Hermann Schägger
Tricine–SDS-PAGE is commonly used to separate proteins in the mass range 1–100 kDa. It is the preferred electrophoretic system for the resolution of proteins smaller than 30 kDa. The concentrations of acrylamide used in the gels are lower than in other electrophoretic systems. These lower concentrations facilitate electroblotting, which is particularly crucial for hydrophobic proteins. Tricine–...
متن کاملBiochemical and electrophoretic analyses of saliva from the predatory reduviid species Rhynocoris marginatus (Fab.).
The saliva of Rhynocoris marginatus consists of amylase, invertase, trehalase, protease, acid phosphatase, alkaline phosphatase, phospholipase, lipase, trypsin, hyaluronidase, and esterase. All enzyme activities were significantly higher in the saliva of female R. marginatus when compared to the saliva of male individuals. The saliva was analyzed by tricine SDS/PAGE, sephadex column chromatogra...
متن کاملDetermination of somatic and excretory-secretory antigens of Fasciola hepatica and Fasciola gigantica using SDS-PAGE
Human fascioliasis due to unknown species and animal fascioliasis caused by both or one of Fasciola spp. are commonly seen in Iran. To compare electrophoretic patterns of somatic and excretory-secretory antigens of F. hepatica and F. gigantica by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), the adult flukes were collected from infected slaughtered bovine livers. E/S a...
متن کاملIdentification of electrophoretic proteins pattern of Fusaium verticillioides isolates from maize by SDS-PAGE.
متن کامل
SDS-PAGE analysis of urinary proteins in dogs with heartworm disease
The aim of the study was to describe the urinary electrophoretic pattern of dogs with heartworm disease.Urine samples from 15 heartworm-infected and 15 healthy dogs were taken. Urinary specific gravity, urinaryprotein concentration and the urine protein/creatinine (U P/C) ratio were determined. Urine proteins werefractionated using SDS-PAGE. Results showed statistically significant differences ...
متن کامل